Fast Lipoprotein Chromatography (FLPC) is a novel method for the quantitation of lipoprotein fractions. Plasma proteins are separated by fast flow gel filtration and lipoproteins are detected after post-column derivatization with an enzymatic cholesterol reagent. FLPC resolves VLDL, LDL, and HDL. Coefficients of variation for determinations of VLDL-, LDL-, and HDL-cholesterol are 5.8%, 2.0%, and 1.9%, respectively. Comparison of FLPC with a combined ultracentrifugation and precipitation method obtained correlation coefficients of 0.983, 0.973, and 0.941 for VLDL-C, LDL-C, and HDL-C, respectively.