Abstract
We describe a high-throughput, automated single-molecule measurement system, equipped with microfluidics. The microfluidic mixing device has integrated valves and pumps to accurately accomplish titration of biomolecules with picoliter resolution. We demonstrate that the approach enabled rapid sampling of biomolecule conformational landscape and of enzymatic activity, in the form of transcription by Escherichia coli RNA polymerase, as a function of the chemical environment.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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DNA-Directed RNA Polymerases / chemistry*
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Escherichia coli / enzymology
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Fluorescence Resonance Energy Transfer / methods*
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High-Throughput Screening Assays*
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Microfluidic Analytical Techniques / instrumentation*
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Protein Conformation
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RNA, Messenger / analysis*
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Transcription, Genetic*
Substances
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RNA, Messenger
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DNA-Directed RNA Polymerases