Simultaneous gas chromatography-tandem mass spectrometry quantification of symmetric and asymmetric dimethylarginine in human urine

Dimitrios Tsikas; Bibiana Beckmann; Frank-Mathias Gutzki; Jens Jordan

doi:10.1016/j.ab.2011.02.002

Simultaneous gas chromatography-tandem mass spectrometry quantification of symmetric and asymmetric dimethylarginine in human urine

Anal Biochem. 2011 Jun 1;413(1):60-2. doi: 10.1016/j.ab.2011.02.002. Epub 2011 Mar 23.

Authors

Dimitrios Tsikas¹, Bibiana Beckmann, Frank-Mathias Gutzki, Jens Jordan

Affiliation

¹ Institute of Clinical Pharmacology, Hannover Medical School, 30625 Hannover, Germany. [email protected]

PMID: 21303648
DOI: 10.1016/j.ab.2011.02.002

Abstract

Previously, we demonstrated the utility of a gas chromatography-tandem mass spectrometry (GC-MS/MS) method for the quantitative determination of asymmetric dimethylarginine (ADMA) in biological samples. Here we report the extension of this method to symmetric dimethylarginine (SDMA) in human urine. SDMA and ADMA were simultaneously quantitated in urine by using their in situ prepared trideuteromethyl esters as internal standards. The GC-MS/MS method was validated for SDMA and ADMA in spot urine samples of 19 healthy adults. In these samples, the creatinine-corrected excretion rate was 3.23±0.63 μmol/mmol for SDMA and 3.14±0.98 μmol/mmol for ADMA.

MeSH terms

Adult
Arginine / analogs & derivatives*
Arginine / urine
Gas Chromatography-Mass Spectrometry / methods*
Humans
Stereoisomerism
Tandem Mass Spectrometry / methods

Substances

symmetric dimethylarginine
N,N-dimethylarginine
Arginine