Abstract
Kinesin-1 is a microtubule plus-end-directed motor that transports various cargos along the axon. Previous studies have elucidated the physical and genetic interactions between kinesin-1 and cytoplasmic dynein, a microtubule minus-end-directed motor, in neuronal cells. However, the physiological importance of kinesin-1 in the dynein-dependent retrograde transport of cargo molecules remains obscure. Here, we show that Caenorhabditis elegans kinesin-1 forms a complex with dynein via its interaction with UNC-16, which binds to the dynein light intermediate (DLI) chain. Both kinesin-1 and UNC-16 are required for localization of DLI-1 at the plus ends of nerve process microtubules. In addition, retrograde transport of APL-1 depends on kinesin-1, UNC-16, and dynein. These results suggest that kinesin-1 mediates the anterograde transport of dynein using UNC-16 as a scaffold and that dynein in turn mediates the retrograde transport of cargo molecules in vivo. Thus, UNC-16 functions as an adaptor for kinesin-1-mediated transport of dynein.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing / physiology*
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Animals
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Animals, Genetically Modified
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Biological Transport
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COS Cells
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Caenorhabditis elegans
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Caenorhabditis elegans Proteins / genetics
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Caenorhabditis elegans Proteins / metabolism
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Caenorhabditis elegans Proteins / physiology*
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Chlorocebus aethiops
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Cytoplasmic Dyneins / genetics
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Cytoplasmic Dyneins / metabolism*
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Dyneins / metabolism
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Gene Expression Regulation / physiology
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Immunoprecipitation / methods
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Kinesins / genetics
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Kinesins / metabolism*
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Luminescent Proteins / genetics
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Luminescent Proteins / metabolism
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Mechanoreceptors / cytology
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Mechanoreceptors / metabolism
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Membrane Proteins / genetics
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Membrane Proteins / metabolism
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Microinjections / methods
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Presynaptic Terminals / metabolism
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Protein Binding
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Protein Transport / genetics
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Transfection / methods
Substances
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APL-1 protein, C elegans
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Adaptor Proteins, Signal Transducing
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Caenorhabditis elegans Proteins
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Luminescent Proteins
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Membrane Proteins
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unc-16 protein, C elegans
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Dli-1 protein, C elegans
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Cytoplasmic Dyneins
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Dyneins
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Kinesins