Abstract
The present study aims to evaluate the combined protective effects of quercetin and α-tocopherol on isoproterenol-treated myocardial infarcted rats. Male albino Wistar rats were pretreated with a combination of quercetin (10 mg/kg) and α-tocopherol (10 mg/kg) daily for 14 days. After the pretreatment, rats were injected isoproterenol (100 mg/kg) to induce myocardial infarction. Isoproterenol-treated rats showed increased levels of serum troponins and increased intensities of serum lactate dehydrogenase-1 and -2 isoenzyme bands. Isoproterenol treatment also showed significant decreased levels of antioxidant system and significant increased levels of plasma lipid peroxidation, plasma uric acid, and the heart calcium. Furthermore, isoproterenol-treated rat's electrocardiogram showed elevated ST segments. Combined pretreatment with quercetin and α-tocopherol normalized all the biochemical parameters and minimized the alterations in electrocardiogram. Histopathology of myocardium also confirmed the cardioprotective effects of quercetin and α-tocopherol. In vitro studies confirmed the mechanism of action of quercetin and α-tocopherol. Thus, quercetin and α-tocopherol exhibited cardioprotective effects against isoproterenol-induced cardiotoxicity due to their scavenging free radicals, improving antioxidants and maintaining Ca(2+) levels. Our study also showed that combined pretreatment (quercetin and α-tocopherol) was highly effective than single pretreatment (quercetin or α-tocopherol).
Copyright © 2010 Wiley Periodicals, Inc.
Publication types
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Research Support, Non-U.S. Gov't
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Retracted Publication
MeSH terms
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Animals
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Antioxidants / metabolism*
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Ascorbic Acid / metabolism
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Calcium / metabolism*
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Cardiotonic Agents / pharmacology*
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Drug Therapy, Combination
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Electrocardiography / methods
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Free Radical Scavengers / pharmacology
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Glutathione / drug effects
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Glutathione / metabolism
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Glutathione Peroxidase / drug effects
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Glutathione Peroxidase / metabolism
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Glutathione Reductase / drug effects
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Glutathione Reductase / metabolism
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Isoenzymes / drug effects
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Isoenzymes / metabolism
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Isoproterenol
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L-Lactate Dehydrogenase / drug effects
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L-Lactate Dehydrogenase / metabolism
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Lipid Peroxidation / drug effects
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Male
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Myocardial Infarction / chemically induced
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Myocardial Infarction / drug therapy*
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Myocardial Infarction / metabolism
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Myocardium / cytology
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Myocardium / metabolism*
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Myocardium / pathology
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Quercetin / pharmacology*
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Rats
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Rats, Wistar
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Superoxide Dismutase / drug effects
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Superoxide Dismutase / metabolism
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Thiobarbituric Acid Reactive Substances / metabolism
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Troponin I / blood
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Troponin I / drug effects
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Troponin I / metabolism
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Troponin T / blood
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Troponin T / drug effects
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Troponin T / metabolism
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Uric Acid / blood
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Uric Acid / metabolism
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alpha-Tocopherol / pharmacology*
Substances
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Antioxidants
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Cardiotonic Agents
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Free Radical Scavengers
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Isoenzymes
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Thiobarbituric Acid Reactive Substances
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Troponin I
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Troponin T
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Uric Acid
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Quercetin
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L-Lactate Dehydrogenase
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lactate dehydrogenase 2
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lactate dehydrogenase 1
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Glutathione Peroxidase
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Superoxide Dismutase
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Glutathione Reductase
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Glutathione
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alpha-Tocopherol
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Isoproterenol
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Ascorbic Acid
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Calcium