The cardioprotective effects of a combination of quercetin and α-tocopherol on isoproterenol-induced myocardial infarcted rats

J Biochem Mol Toxicol. 2011 Jan-Feb;25(1):28-40. doi: 10.1002/jbt.20357.

Abstract

The present study aims to evaluate the combined protective effects of quercetin and α-tocopherol on isoproterenol-treated myocardial infarcted rats. Male albino Wistar rats were pretreated with a combination of quercetin (10 mg/kg) and α-tocopherol (10 mg/kg) daily for 14 days. After the pretreatment, rats were injected isoproterenol (100 mg/kg) to induce myocardial infarction. Isoproterenol-treated rats showed increased levels of serum troponins and increased intensities of serum lactate dehydrogenase-1 and -2 isoenzyme bands. Isoproterenol treatment also showed significant decreased levels of antioxidant system and significant increased levels of plasma lipid peroxidation, plasma uric acid, and the heart calcium. Furthermore, isoproterenol-treated rat's electrocardiogram showed elevated ST segments. Combined pretreatment with quercetin and α-tocopherol normalized all the biochemical parameters and minimized the alterations in electrocardiogram. Histopathology of myocardium also confirmed the cardioprotective effects of quercetin and α-tocopherol. In vitro studies confirmed the mechanism of action of quercetin and α-tocopherol. Thus, quercetin and α-tocopherol exhibited cardioprotective effects against isoproterenol-induced cardiotoxicity due to their scavenging free radicals, improving antioxidants and maintaining Ca(2+) levels. Our study also showed that combined pretreatment (quercetin and α-tocopherol) was highly effective than single pretreatment (quercetin or α-tocopherol).

Publication types

  • Research Support, Non-U.S. Gov't
  • Retracted Publication

MeSH terms

  • Animals
  • Antioxidants / metabolism*
  • Ascorbic Acid / metabolism
  • Calcium / metabolism*
  • Cardiotonic Agents / pharmacology*
  • Drug Therapy, Combination
  • Electrocardiography / methods
  • Free Radical Scavengers / pharmacology
  • Glutathione / drug effects
  • Glutathione / metabolism
  • Glutathione Peroxidase / drug effects
  • Glutathione Peroxidase / metabolism
  • Glutathione Reductase / drug effects
  • Glutathione Reductase / metabolism
  • Isoenzymes / drug effects
  • Isoenzymes / metabolism
  • Isoproterenol
  • L-Lactate Dehydrogenase / drug effects
  • L-Lactate Dehydrogenase / metabolism
  • Lipid Peroxidation / drug effects
  • Male
  • Myocardial Infarction / chemically induced
  • Myocardial Infarction / drug therapy*
  • Myocardial Infarction / metabolism
  • Myocardium / cytology
  • Myocardium / metabolism*
  • Myocardium / pathology
  • Quercetin / pharmacology*
  • Rats
  • Rats, Wistar
  • Superoxide Dismutase / drug effects
  • Superoxide Dismutase / metabolism
  • Thiobarbituric Acid Reactive Substances / metabolism
  • Troponin I / blood
  • Troponin I / drug effects
  • Troponin I / metabolism
  • Troponin T / blood
  • Troponin T / drug effects
  • Troponin T / metabolism
  • Uric Acid / blood
  • Uric Acid / metabolism
  • alpha-Tocopherol / pharmacology*

Substances

  • Antioxidants
  • Cardiotonic Agents
  • Free Radical Scavengers
  • Isoenzymes
  • Thiobarbituric Acid Reactive Substances
  • Troponin I
  • Troponin T
  • Uric Acid
  • Quercetin
  • L-Lactate Dehydrogenase
  • lactate dehydrogenase 2
  • lactate dehydrogenase 1
  • Glutathione Peroxidase
  • Superoxide Dismutase
  • Glutathione Reductase
  • Glutathione
  • alpha-Tocopherol
  • Isoproterenol
  • Ascorbic Acid
  • Calcium