Evaluation of a DNA microarray (Check-MDR CT102) for rapid detection of TEM, SHV, and CTX-M extended-spectrum β-lactamases and of KPC, OXA-48, VIM, IMP, and NDM-1 carbapenemases

J Clin Microbiol. 2011 Apr;49(4):1608-13. doi: 10.1128/JCM.02607-10. Epub 2011 Feb 16.

Abstract

The Check-MDR CT102 microarray, aimed at identifying bacteria producing extended-spectrum β-lactamase (ESBL) (SHV, TEM, and CTX-M) and carbapenemase (KPC, OXA-48, VIM, IMP, and NDM-1), was evaluated on a total of 144 Gram-negative strains expressing various β-lactamases. The sensitivity and specificity were 100% for most tested genes, suggesting that this assay allows accurate identification of common ESBL and carbapenemase producers from bacterial cultures.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Gram-Negative Bacteria / enzymology*
  • Gram-Negative Bacteria / genetics
  • Gram-Negative Bacteria / isolation & purification
  • Humans
  • Microbial Sensitivity Tests / methods
  • Oligonucleotide Array Sequence Analysis / methods*
  • Sensitivity and Specificity
  • beta-Lactamases / classification
  • beta-Lactamases / genetics*

Substances

  • beta-Lactamases