Artificial microRNAs (amiRNAs) engineering - On how microRNA-based silencing methods have affected current plant silencing research

Gaurav Sablok; Alvaro L Pérez-Quintero; Mehedi Hassan; Tatiana V Tatarinova; Camilo López

doi:10.1016/j.bbrc.2011.02.045

Artificial microRNAs (amiRNAs) engineering - On how microRNA-based silencing methods have affected current plant silencing research

Biochem Biophys Res Commun. 2011 Mar 18;406(3):315-9. doi: 10.1016/j.bbrc.2011.02.045. Epub 2011 Feb 15.

Authors

Gaurav Sablok¹, Alvaro L Pérez-Quintero, Mehedi Hassan, Tatiana V Tatarinova, Camilo López

Affiliation

¹ Key Lab of Horticultural Plant Biology (MOE), Huazhong Agricultural University, Shizishan, Wuhan 430070, China. [email protected]

PMID: 21329663
DOI: 10.1016/j.bbrc.2011.02.045

Abstract

In recent years, endogenous microRNAs have been described as important regulators of gene expression in eukaryotes. Artificial microRNAs (amiRNAs) represent a recently developed miRNA-based strategy to silence endogenous genes. amiRNAs can be created by exchanging the miRNA/miRNA(∗) sequence within a miRNA precursor with a sequence designed to match the target gene, this is possible as long as the secondary RNA structure of the precursor is kept intact. In this review, we summarize the basic methodologies to design amiRNAs and detail their applications in plants genetic functional studies as well as their potential for crops genetic improvement.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Crops, Agricultural / genetics*
Gene Expression Regulation, Plant*
Gene Silencing*
Genetic Engineering*
MicroRNAs / genetics*
Plants / genetics*
Plants / immunology
Plants, Genetically Modified / genetics*
Transformation, Genetic

Substances

MicroRNAs