The electrical property of graphene is highly sensitive to its local environment, which makes graphene an ideal channel material in electronic sensors. Reduced graphene oxide (rGO) has been used as the desirable alternative to the pristine graphene due to its low-cost, solution-processable, and scalable production. In this paper, we present a field-effect transistor sensor using micropatterned, protein-functionalized rGO film as the conducting or sensing channel. Such a nanoelectronic sensor is able to detect various metal ions in real-time with high sensitivity.