Objective: To study the chemical constituents from the rhizome of Paris axialis.
Method: The compounds were isolated by column chromatography with silica gel and purified by Sephadex LH-20 column chromatography and preparative RP-HPLC. The structures were identified by means of spectroscopic methods.
Result: Fourteen compounds were isolated from the EtOAc extract and the n-BuOH extract of P. axialis. Their structures were identified as daucosterol (1), stigmasterol-3-O-beta-D-glycopyranoside (2), beta-ecdysterone (3), pennogenin-3-O-alpha-L-arabinofuranosyl (1 --> 4) -[alpha-L -rhamnopyranosyl (1 --> 2)] -beta-D-glycopyranoside (4), diosgenin-3-O-alpha-L- rhamnopy-ranosyl (1 --> 4) -alpha-L-rhamnopyranosyl (1 --> 4) [alpha-L-rhamnopyranosyl (1 --> 2)] -beta-D-glycopyranoside (5), kaempferol (6), rutin (7), myrincitrin (8), 4, 2', 4'-trihydroxychalcone (9), isorhamnetin-3-O-beta-D- glycopyranoside (10), isorhamnetin-3-O-alpha-L-rhamnopyranosyl (1 --> 2) -beta-D-glycopyranoside (11), isorhamnetin-3-O-beta-D-glucpyranosyl (1 --> 6) -beta-D-glycopyranoside (12), kayaflavone (13), amentoflavone (14).
Conclusion: Compounds 1-3 and 6-14 are isolated from P. axialis for the first time; and compounds 7-10, 13, 14 are isolated from the genus Paris for the first time.