Abstract
Trophoblast stem (TS) cells are ideal models to investigate trophectoderm differentiation and placental development. Herein, we describe the derivation of rabbit trophoblast stem cells from embryonic stem (ES) cells. Rabbit ES cells generated in our laboratory were induced to differentiate in the presence of BMP4 and TS-like cell colonies were isolated and expanded. These cells expressed the molecular markers of mouse TS cells, were able to invade, give rise to derivatives of TS cells, and chimerize placental tissues when injected into blastocysts. The rabbit TS-like cells maintained self-renewal in culture medium with serum but without growth factors or feeder cells, whilst their proliferation and identity were compromised by inhibitors of FGFs and TGF-β receptors. Taken together, our study demonstrated the derivation of rabbit TS cells and suggested the essential roles of FGF and TGF-β signalings in maintenance of rabbit TS cell self-renewal.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bone Morphogenetic Protein 4 / pharmacology*
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Cell Differentiation / drug effects*
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Cell Differentiation / genetics
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Cells, Cultured
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Embryoid Bodies / cytology
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Embryoid Bodies / drug effects
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Embryoid Bodies / metabolism
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Embryoid Bodies / physiology
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Embryonic Stem Cells / drug effects*
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Embryonic Stem Cells / metabolism
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Embryonic Stem Cells / physiology*
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Epithelial Cells / drug effects
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Epithelial Cells / metabolism
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Epithelial Cells / physiology
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Female
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Gene Expression Regulation
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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Mice
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Organ Specificity / genetics
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Organisms, Genetically Modified
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Pregnancy
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Rabbits
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Stem Cells / cytology
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Stem Cells / drug effects
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Stem Cells / metabolism
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Stem Cells / physiology
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Trophoblasts / cytology
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Trophoblasts / drug effects
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Trophoblasts / metabolism
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Trophoblasts / physiology*
Substances
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Bmp4 protein, mouse
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Bone Morphogenetic Protein 4
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Green Fluorescent Proteins