MicroRNA-16 targets zyxin and promotes cell motility in human laryngeal carcinoma cell line HEp-2

IUBMB Life. 2011 Feb;63(2):101-8. doi: 10.1002/iub.417. Epub 2011 Feb 24.

Abstract

MicroRNAs are small noncoding RNAs that negatively regulate target mRNAs at the posttranscription level. Here, we show that miR-16 is upregulated in human laryngeal carcinoma tissues. Inhibition of miR-16 in HEp-2 laryngeal cancer cell line could suppress cell migration and enhance cell-cell adhesion. Subsequently, zyxin, whose expression is negatively regulated by miR-16, is confirmed to be a direct target gene of miR-16. Furthermore, overexpression of zyxin could also restrain cell movement and enhance cell-cell adhesion. The study of miR-16 and its target zyxin will shed light on diagnosis and therapy of laryngeal cancer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers, Tumor / analysis
  • Cell Adhesion / genetics
  • Cell Line, Tumor
  • Cell Movement / genetics
  • Cell Proliferation
  • Cytoskeletal Proteins* / genetics
  • Cytoskeletal Proteins* / metabolism
  • Down-Regulation
  • Gene Expression Regulation, Neoplastic
  • Glycoproteins* / genetics
  • Glycoproteins* / metabolism
  • Humans
  • Laryngeal Neoplasms / diagnosis
  • Laryngeal Neoplasms / genetics*
  • Laryngeal Neoplasms / metabolism*
  • Laryngeal Neoplasms / pathology
  • MicroRNAs* / genetics
  • MicroRNAs* / metabolism
  • Organ Specificity
  • Transformation, Genetic
  • Up-Regulation
  • Zyxin

Substances

  • Biomarkers, Tumor
  • Cytoskeletal Proteins
  • Glycoproteins
  • MicroRNAs
  • ZYX protein, human
  • Zyxin