Cytotoxic effect of hyperthermia and chemotherapy with platinum salt on ovarian cancer cells: results of an in vitro study

Eur Surg Res. 2011;46(3):139-47. doi: 10.1159/000324395. Epub 2011 Mar 4.

Abstract

Purpose: Hyperthermic intraperitoneal chemotherapy is continuously under evaluation in ovarian cancer. The purpose of the present study was to evaluate the effect of chemotherapy, drug concentration and temperature.

Materials and methods: A human ovarian carcinoma cell line was used. The effect of hyperthermia combined with chemotherapy was analyzed.

Results: When hyperthermia was combined with chemotherapy, the 50% lethal dose (LD(50)) decreased with the duration of exposure. The effect of temperature was similar between 39 and 43 °C for a 30-min exposure. For a 60- to 90-min exposure, the LD(50) was equivalent between 38 and 43 °C. Beyond 40 °C, an increase in platinum salt concentration was necessary to obtain similar results according to the duration of exposure.

Conclusions: The cytotoxic effect of the combination seemed to be potentiated and limited at 40 °C.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents / administration & dosage
  • Antineoplastic Agents / therapeutic use*
  • Carboplatin / administration & dosage
  • Carboplatin / therapeutic use*
  • Cell Line, Tumor
  • Cell Survival / drug effects
  • Cisplatin / administration & dosage
  • Cisplatin / therapeutic use*
  • Combined Modality Therapy
  • Dose-Response Relationship, Drug
  • Drug Resistance, Neoplasm
  • Female
  • Humans
  • Hyperthermia, Induced*
  • Ovarian Neoplasms / drug therapy*
  • Ovarian Neoplasms / pathology
  • Ovarian Neoplasms / therapy*

Substances

  • Antineoplastic Agents
  • Carboplatin
  • Cisplatin