Abstract
The purpose of this study was to analyze the expression of B8.7 antigen and its implication in the low molecular weight B-Cell growth factor (LMW BCGF) proliferative pathway at the early stages of the human B-cell differentiation. After an overnight incubation in culture medium of B-cell precursor acute lymphoblastic leukemias (ALL), we demonstrated the presence of B8.7 antigen in 18 of 25 cases (72%). Such an incubation also induced a significant increase in the LMW BCGF responsiveness of ALL cells (P less than 0.03). In addition, we showed a significant correlation between B8.7 expression and the ability of pre-B ALL cells to respond to LMW BCGF. As previously described for normal B cells, the anti-B8.7 monoclonal antibody inhibited the LMW BCGF-dependent proliferation of pre-B ALL cells in a dose-dependent manner. These data indicate that B8.7 antigen is expressed and may be functionally related to the LMW BCGF pathway at the pre-B cell stages of differentiation. These results also suggest that human B-cell precursor ALL are not only phenotypically similar to their normal B lymphocyte counterparts, but are also sensitive to the same immunoregulatory cytokines that control normal cell growth.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adolescent
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Adult
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Aged
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Antibodies, Monoclonal / immunology
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Antigens, Differentiation / metabolism
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Antigens, Differentiation, B-Lymphocyte / immunology*
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Antigens, Differentiation, B-Lymphocyte / metabolism
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Antigens, Neoplasm / immunology*
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Antigens, Neoplasm / metabolism
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Cell Transformation, Neoplastic / drug effects
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Child
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Child, Preschool
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Female
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Humans
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Lymphokines / metabolism
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Lymphokines / pharmacology
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Lymphotoxin-alpha / pharmacology
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Male
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Middle Aged
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Neprilysin
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Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / immunology*
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Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / metabolism
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Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / pathology
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Precursor Cell Lymphoblastic Leukemia-Lymphoma / immunology*
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Precursor Cell Lymphoblastic Leukemia-Lymphoma / metabolism
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Precursor Cell Lymphoblastic Leukemia-Lymphoma / pathology
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Receptors, Fc / immunology
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Receptors, Fc / metabolism
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Receptors, IgE
Substances
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Antibodies, Monoclonal
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Antigens, Differentiation
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Antigens, Differentiation, B-Lymphocyte
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Antigens, Neoplasm
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B8.7 antigen, human
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Lymphokines
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Lymphotoxin-alpha
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Receptors, Fc
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Receptors, IgE
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low molecular weight-B cell growth factor
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Neprilysin