Abstract
A large number of cell lines which attach and spread on laminin show a comparable binding either to both laminin fragments P1 and E8 or exclusively to E8. Adhesion to fragment E8 was with one exception completely inhibited by a monoclonal antibody to the alpha 6 integrin subunit, indicating that VLA-6 or a related structure is the major cellular receptor for laminin. It is not involved in fragment P1 adhesion. Synthetic peptides possessing RGD or YIGSR sequences were without inhibitory activity for alpha 6-mediated adhesion to fragment E8.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Amino Acids / analysis
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Animals
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Antibodies, Monoclonal / immunology*
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Antibody Specificity / immunology*
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Cell Adhesion / drug effects
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Fibroblasts / drug effects*
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Fibroblasts / metabolism
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Fibroblasts / physiology
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Humans
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Integrins / immunology*
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Laminin / analysis
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Laminin / metabolism*
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Mice
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Molecular Sequence Data
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Neoplasms / metabolism
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Neoplasms / pathology
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Neoplasms / physiopathology
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Rats
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Receptors, Immunologic / metabolism*
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Receptors, Laminin
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Tumor Cells, Cultured / metabolism
Substances
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Amino Acids
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Antibodies, Monoclonal
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Integrins
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Laminin
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Receptors, Immunologic
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Receptors, Laminin