Ultrasensitive endonuclease activity and inhibition detection using gold nanoparticle-enhanced fluorescence polarization

Yong Huang; Shulin Zhao; Zhen-Feng Chen; Yan-Cheng Liu; Hong Liang

doi:10.1039/c1cc10325c

Ultrasensitive endonuclease activity and inhibition detection using gold nanoparticle-enhanced fluorescence polarization

Chem Commun (Camb). 2011 Apr 28;47(16):4763-5. doi: 10.1039/c1cc10325c. Epub 2011 Mar 11.

Authors

Yong Huang¹, Shulin Zhao, Zhen-Feng Chen, Yan-Cheng Liu, Hong Liang

Affiliation

¹ Ministry of Education Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin, Guangxi 541004, China.

PMID: 21399806
DOI: 10.1039/c1cc10325c

Abstract

We have developed a highly sensitive and selective fluorescence polarization assay method based on the specificity of the DNA cleavage reaction with the enhancement of gold nanoparticles (AuNPs) for assaying endonuclease activity and inhibition. This assay can detect EcoRI endonuclease down to 5.0×10(-4) U mL(-1) with a detection range from 5.0×10(-4) to 10 U mL(-1).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Deoxyribonuclease EcoRI / antagonists & inhibitors*
Deoxyribonuclease EcoRI / chemistry
Deoxyribonuclease EcoRI / metabolism*
Enzyme Activation / drug effects
Enzyme Inhibitors / pharmacology*
Fluorescence Polarization*
Gold / chemistry*
Metal Nanoparticles / chemistry*

Substances

Enzyme Inhibitors
Gold
Deoxyribonuclease EcoRI