Vitrification at the germinal vesicle stage does not affect the methylation profile of H19 and KCNQ1OT1 imprinting centers in human oocytes subsequently matured in vitro

Fertil Steril. 2011 May;95(6):1955-60. doi: 10.1016/j.fertnstert.2011.02.029. Epub 2011 Mar 21.

Abstract

Objective: To evaluate the integrity of genomic imprinting in oocytes vitrified at the germinal vesicle (GV) stage and in vitro matured (IVM) after thawing.

Design: Clinical research and application.

Setting: University-based fertility center.

Patient(s): Immature oocytes were donated for research by patients who were included in an intracytoplasmic sperm injection program.

Intervention(s): Immature oocyte retrieval after ovarian stimulation, followed by oocyte vitrification, thawing, and IVM.

Main outcome measure(s): Methylation profile of H19 and KCNQ1OT1 imprinting control regions, H19DMR and KvDMR1, respectively.

Result(s): Among 184 vitrified GV oocytes, 102 survived thawing (55.4%), 77 (75.5%) of which reached the meiosis II (MII) stage after IVM. One hundred twenty control GV oocytes were only subjected to IVM; 70.8% reached the MII stage. GV vitrified as well as control oocytes acquired full imprint at KvDMR1 after IVM and generally retained the unmethylated state of H19DMR.

Conclusion(s): For the first time, we show that oocyte vitrification does not affect the methylation profile of H19DMR and KvDMR1: during their IVM, vitrified GV oocytes acquire DNA methylation in the maternally imprinted KCNQ1OT1 gene with the same efficiency as fresh GV oocytes; the vitrification process does not alter the unmethylated state of the paternally imprinted H19 gene.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Cells, Cultured
  • Cleavage Stage, Ovum / metabolism
  • Cleavage Stage, Ovum / physiology*
  • Cryopreservation
  • DNA Methylation* / physiology
  • Female
  • Genomic Imprinting / physiology
  • Humans
  • Oocytes*
  • Oogenesis / genetics
  • Oogenesis / physiology
  • Potassium Channels, Voltage-Gated / genetics
  • Potassium Channels, Voltage-Gated / metabolism
  • RNA, Long Noncoding
  • RNA, Untranslated / genetics*
  • RNA, Untranslated / metabolism
  • Vitrification*
  • Young Adult

Substances

  • H19 long non-coding RNA
  • KCNQ1OT1 long non-coding RNA, human
  • Potassium Channels, Voltage-Gated
  • RNA, Long Noncoding
  • RNA, Untranslated