Antioxidant and antiglycating activities of Salvia sahendica and its protective effect against oxidative stress in neuron-like PC12 cells

J Nat Med. 2011 Jul;65(3-4):455-65. doi: 10.1007/s11418-011-0519-9. Epub 2011 Mar 18.

Abstract

Increased oxidative stress is widely accepted to be a factor in the development and progression of Alzheimer's disease (AD). Here we introduced Salvia sahendica as a protective agent in differentiated PC12 cells, which are commonly considered to be a reliable model of neuronal cells. Our results demonstrated that S. sahendica has antioxidant and antiglycating properties in in vitro system and these properties are expanded into H(2)O(2)-induced model. S. sahendica inhibited H(2)O(2)-induced cell death in PC12 cells. We further showed that this plant exerts its protective effect by increasing superoxide dismutase and catalase levels, reducing lipid peroxidation and upregulating hemoxygenase-1 and glutamylcysteine synthetase proteins. This study raises the possibility of developing S. sahendica as a potential neuroprotective agent.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / chemistry
  • Antioxidants / pharmacology*
  • Catalase / metabolism
  • Cell Death / drug effects
  • Chromatography, High Pressure Liquid
  • Hydrogen Peroxide / pharmacology
  • Lipid Peroxidation / drug effects
  • Oxidative Stress / drug effects*
  • PC12 Cells
  • Plant Extracts / chemistry
  • Plant Extracts / pharmacology*
  • Rats
  • Salvia / chemistry*
  • Superoxide Dismutase / metabolism

Substances

  • Antioxidants
  • Plant Extracts
  • Hydrogen Peroxide
  • Catalase
  • Superoxide Dismutase