Idiomarina maris sp. nov., a marine bacterium isolated from sediment

Int J Syst Evol Microbiol. 2012 Feb;62(Pt 2):370-375. doi: 10.1099/ijs.0.027896-0. Epub 2011 Mar 25.

Abstract

A protease-producing marine bacterium, designated CF12-14(T), was isolated from sediment of the South China Sea. Phylogenetic analysis of the 16S rRNA gene sequence revealed that strain CF12-14(T) formed a separate lineage within the genus Idiomarina (Gammaproteobacteria). The isolate showed the highest 16S rRNA gene sequence similarity with Idiomarina salinarum ISL-52(T) (94.7 %), Idiomarina seosinensis CL-SP19(T) (94.6 %) and other members of the genus Idiomarina (91.9-94.6 %). Cells were gram-negative, aerobic, flagellated, straight or slightly curved, and often formed buds and prosthecae. Strain CF12-14(T) grew at 4-42 °C (optimum 30-35 °C) and with 0.1-15 % (w/v) NaCl (optimum 2-3 %). The isolate reduced nitrate to nitrite and hydrolysed DNA, but did not produce acids from sugars. The predominant cellular fatty acids were iso-C(15 : 0) (27.4 %), iso-C(17 : 0) (16.0 %) and iso-C(17 : 1)ω9c (15.8 %). The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The major respiratory quinone was ubiquinone 8. The DNA G+C content was 50.4 mol%. The phylogenetic, phenotypic and chemotaxonomic data supported the conclusion that CF12-14(T) represents a novel species of the genus Idiomarina, for which the name Idiomarina maris sp. nov. is proposed. The type strain is CF12-14(T) ( = CCTCC AB 208166(T) = KACC 13974(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alteromonadaceae / chemistry
  • Alteromonadaceae / classification*
  • Alteromonadaceae / genetics
  • Alteromonadaceae / isolation & purification*
  • Bacterial Typing Techniques
  • DNA, Bacterial / analysis
  • DNA, Ribosomal / analysis
  • Fatty Acids / analysis
  • Genes, rRNA
  • Geologic Sediments / microbiology*
  • Lipids / analysis
  • Molecular Sequence Data
  • Phenotype
  • Phylogeny
  • Quinones / analysis
  • RNA, Ribosomal, 16S / genetics
  • Seawater / microbiology*
  • Sequence Analysis, DNA
  • Species Specificity

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • Fatty Acids
  • Lipids
  • Quinones
  • RNA, Ribosomal, 16S

Associated data

  • GENBANK/EU723884