Abstract
Sphingosine 1-phosphate receptor 1 (S1P(1)) is critical for lymphocyte recirculation and is a clinical target for treatment of multiple sclerosis. By generating a short-duration S1P(1) agonist and mice in which fluorescently tagged S1P(1) replaces wild-type receptor, we elucidate physiological and agonist-perturbed changes in expression of S1P(1) at a subcellular level in vivo. We demonstrate differential downregulation of S1P(1) on lymphocytes and endothelia after agonist treatment.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Animals
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Down-Regulation / drug effects
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Endothelium / drug effects
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Endothelium / metabolism
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Flow Cytometry
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Fluorescent Dyes / chemistry
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Fluorescent Dyes / metabolism
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Gene Knock-In Techniques*
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Green Fluorescent Proteins / chemistry*
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Green Fluorescent Proteins / metabolism
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Lymphocytes / drug effects
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Lymphocytes / metabolism
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Mice
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Multiple Sclerosis / drug therapy*
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Multiple Sclerosis / metabolism
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Multiple Sclerosis / pathology
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Receptors, Lysosphingolipid / agonists*
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Receptors, Lysosphingolipid / metabolism
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Receptors, Lysosphingolipid / therapeutic use*
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Time Factors
Substances
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Fluorescent Dyes
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Receptors, Lysosphingolipid
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enhanced green fluorescent protein
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Green Fluorescent Proteins
Associated data
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PubChem-Substance/112629136
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PubChem-Substance/112629137
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PubChem-Substance/112629138
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PubChem-Substance/112629139
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PubChem-Substance/112629140