Abstract
Background:
The present study determined type I interferon (IFN) receptor (interferon-α/β receptor (IFNAR)) and its predicable role in interferon-α2b treatment in chronic hepatitis B (CHB) patients.
Methods:
Expression of IFN-α/βR-1 and IFN-α/βR-2 in peripheral blood mononuclear cells and in liver tissue was measured by flow cytometry, immunofluorescence, immunohistochemistry and RT-PCR.
Results:
IFN-α/βR-1 and IFN-α/βR-2 in monocytes and lymphocytes increased in CHB patients. Expression of IFNAR-1 and IFNAR-2 in liver had positive correlation with HBV-DNA in liver tissue. Expression of IFN-α/βR in lymphocytes and monocytes increased in the first month, but then decreased during the subsequent interferon-α2b treatment, patients who had higher levels of IFN-α/βR-2 in monocytes prior to therapy showed better viral response than those with lower levels.
Conclusions:
Expression of IFN-α/βR-2 in monocytes can be used as a predictable parameter to evaluate the effect of IFN-α treatment in CHB patients.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Case-Control Studies
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DNA, Viral / blood*
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DNA, Viral / immunology
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Female
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Flow Cytometry
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Fluorescent Antibody Technique
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Hepatitis B Surface Antigens / blood
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Hepatitis B e Antigens / blood
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Hepatitis B virus / drug effects*
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Hepatitis B virus / immunology
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Hepatitis B virus / physiology
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Hepatitis B, Chronic* / blood
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Hepatitis B, Chronic* / drug therapy
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Hepatitis B, Chronic* / immunology
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Hepatitis B, Chronic* / pathology
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Hepatitis B, Chronic* / virology
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Humans
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Immunohistochemistry
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Interferon-alpha / administration & dosage*
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Interferon-alpha / therapeutic use
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Leukocytes, Mononuclear / cytology
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Leukocytes, Mononuclear / drug effects*
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Leukocytes, Mononuclear / immunology
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Male
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Middle Aged
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Receptor, Interferon alpha-beta* / blood
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Receptor, Interferon alpha-beta* / genetics
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Receptor, Interferon alpha-beta* / immunology
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Reverse Transcriptase Polymerase Chain Reaction
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Viral Load / drug effects
Substances
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DNA, Viral
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Hepatitis B Surface Antigens
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Hepatitis B e Antigens
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Interferon-alpha
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Receptor, Interferon alpha-beta