Synchrotron radiation infrared microspectroscopy to assess the activity of vancomycin against endocarditis vegetation bacteria

J Microbiol Methods. 2011 Jun;85(3):235-8. doi: 10.1016/j.mimet.2011.03.013. Epub 2011 Apr 5.

Abstract

Infrared microspectroscopy was used to show that vancomycin alters infrared spectra of endocarditis vegetation bacteria, and that vancomycin effects on bacterial biochemical contents are unevenly distributed between peripheral and central areas of bacterial masses. Infrared microspectroscopy is useful to study the activity of antibacterial agents against bacteria in tissues.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • Bacteria / drug effects*
  • Bacteria / growth & development
  • Bacteria / metabolism
  • Biomass
  • Endocarditis / microbiology*
  • Microbial Sensitivity Tests / methods*
  • Radiation
  • Spectrophotometry, Infrared / methods*
  • Synchrotrons
  • Vancomycin / pharmacology*

Substances

  • Anti-Bacterial Agents
  • Vancomycin