Exclusively NOESY-based automated NMR assignment and structure determination of proteins

J Biomol NMR. 2011 Jun;50(2):137-46. doi: 10.1007/s10858-011-9502-8. Epub 2011 Mar 30.

Abstract

A fully automated method is presented for determining NMR solution structures of proteins using exclusively NOESY spectra as input, obviating the need to measure any spectra only for obtaining resonance assignments but devoid of structural information. Applied to two small proteins, the approach yielded structures that coincided closely with conventionally determined structures.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / chemistry*
  • Computer Simulation
  • Models, Molecular*
  • Nuclear Magnetic Resonance, Biomolecular*
  • Protein Conformation*
  • Reproducibility of Results

Substances

  • Bacterial Proteins