Effect of preexisting immunity to adenovirus on transgene product-specific genital T cell responses on vaccination of mice with a homologous vector

J Infect Dis. 2011 Apr 15;203(8):1073-81. doi: 10.1093/infdis/jiq161.

Abstract

We evaluated changes in global and human immunodeficiency virus (HIV)-specific genital T cells after vaccination of female mice with a replication-defective adenovirus vector of human serotype 5 (AdHu5) expressing Gag protein of HIV-1, in the presence or absence of preexisting immunity to the vaccine carrier. Our data show that preexisting immunity causes a rapid and transient decrease of genital CD4(+) T cells without increasing the expression of chemokine (C-C motif) receptor 5. Furthermore, preexposure to AdHu5 affects long-term alterations in the magnitude and quality of vaccine-induced Gag-specific CD8(+) T cell responses. AdHu5-specific antibodies interfere with the induction of transgene product-specific CD8(+) T cell responses in systemic compartments, whereas some mechanism other than antibodies also seems to affect those that home to the genital tract.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adenoviridae / immunology*
  • Animals
  • CD4-Positive T-Lymphocytes / physiology*
  • CD8-Positive T-Lymphocytes / physiology*
  • Female
  • HIV-1 / immunology*
  • Mice
  • Mice, Inbred BALB C
  • Vaccination
  • Vagina / immunology
  • Viral Vaccines / immunology*
  • gag Gene Products, Human Immunodeficiency Virus / immunology*

Substances

  • Viral Vaccines
  • gag Gene Products, Human Immunodeficiency Virus