[Sequence analysis of the phosphoprotein gene of peste des petits ruminants virus of Chinese origin]

Bing Du Xue Bao. 2011 Jan;27(1):26-33.
[Article in Chinese]

Abstract

The nucleotide sequences of P gene from a field strain of peste des petits ruminants virus (PPRV) ("China/Tib/Gej/07-30") was firstly determined. The P gene is 1,655 nucleotides long with two overlapping open reading frames (ORFs). The first ORF is 1530 nucleotides long and would produce P protein of 509 amino acid residues. The second ORF is 534 nucleotides long and would produce C protein of 177 amino acid residues. The first ORF produces a second mRNA transcript of 897 nucleotides long with an extra G nucleotide at position 751. Translation from this mRNA would produce V protein of 298 amino acid residues. The nucleotide and deduced amino acid sequence were compared with the homologous region of other PPRV isolates. At the amino acid level, the "China/Tib/Gej/07-30" shares homology of 86.10%-97.3%, 84.3%-94.9%, and 82.9%-96.3% for P, C, and V proteins respectively. Several sequence motifs in the P genes were identified on the basis of conservation in the PPRVs and the morbilliviruses.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • China
  • Female
  • Goat Diseases / virology*
  • Goats
  • Molecular Sequence Data
  • Peste-des-Petits-Ruminants / veterinary*
  • Peste-des-Petits-Ruminants / virology
  • Peste-des-petits-ruminants virus / chemistry
  • Peste-des-petits-ruminants virus / genetics*
  • Peste-des-petits-ruminants virus / isolation & purification
  • Peste-des-petits-ruminants virus / metabolism
  • Phosphoproteins / chemistry
  • Phosphoproteins / genetics*
  • Phosphoproteins / metabolism
  • Sequence Analysis
  • Sequence Homology, Amino Acid
  • Viral Proteins / chemistry
  • Viral Proteins / genetics*
  • Viral Proteins / metabolism

Substances

  • Phosphoproteins
  • Viral Proteins