Synthesis and transfection activity of novel galactosylated polycationic lipid

Bioorg Med Chem Lett. 2011 May 15;21(10):2937-40. doi: 10.1016/j.bmcl.2011.03.056. Epub 2011 Mar 21.

Abstract

In this study, we synthesized a new galactosylated cationic lipid and investigated its biological activity. The structure of lipid combines both spermine residue for DNA compaction and galactose moiety for the improvement of aggregation behavior of lipoplexes. Lipid was low toxic for different mammalian cells, and was able both to compact plasmid DNA and to mediate cellular accumulation of various nucleic acids (ODN, pDNA and siRNA) exhibiting biological activity (transgene expression, gene silencing).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Cell Survival / drug effects
  • Cricetinae
  • Flow Cytometry
  • Galactose / chemistry*
  • Gene Transfer Techniques
  • Humans
  • Lipids / chemical synthesis*
  • Lipids / chemistry
  • Lipids / toxicity
  • Liposomes / chemistry
  • Microscopy, Atomic Force
  • Molecular Structure
  • Polyamines / chemistry*
  • Polyelectrolytes
  • Transfection

Substances

  • Lipids
  • Liposomes
  • Polyamines
  • Polyelectrolytes
  • polycations
  • Galactose