Abstract
Methylelaiophylin isolated from Streptomyces melanosporofaciens was selected as an alpha-glucosidase inhibitor with an IC50 value of 10 micrometer. It showed mixedtype inhibition of alpha-glucosidase with a Ki value of 5.94 micrometer. In addition, methylelaiophylin inhibited the intracellular trafficking of hemagglutinin-neuramidase (HN), a glycoprotein of Newcastle disease virus (NDV), in baby hamster kidney (BHK) cells. Methylelaiophylin inhibited the cell surface expression of NDV-HN glycoprotein without significantly affecting HN glycoprotein synthesis in NDV-infected BHK cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antiviral Agents / isolation & purification
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Antiviral Agents / pharmacology*
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Cells, Cultured
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Cricetinae
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Glycoside Hydrolase Inhibitors*
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HN Protein / metabolism
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Inhibitory Concentration 50
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Macrolides / isolation & purification
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Macrolides / pharmacology*
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Newcastle disease virus / drug effects*
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Newcastle disease virus / growth & development
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Protein Transport / drug effects
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Streptomyces / chemistry*
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Streptomyces / metabolism
Substances
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Antiviral Agents
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Glycoside Hydrolase Inhibitors
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HN Protein
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Macrolides
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elaiophylin