Partially purified extract and viscolin from Viscum coloratum attenuate airway inflammation and eosinophil infiltration in ovalbumin-sensitized mice

J Ethnopharmacol. 2011 Jun 1;135(3):646-53. doi: 10.1016/j.jep.2011.03.065. Epub 2011 Apr 5.

Abstract

Aim of the study: Viscum coloratum Nakai is used in traditional Chinese medicine to treat various diseases, including hemorrhage, hypertension, and inflammatory diseases. A previous study demonstrated a partially purified extract (PPE-SVC) and viscolin from Viscum coloratum Nakai inhibited phosphodiesterase activity. In this study, we evaluated the anti-asthmatic effects of PPE-SVC and viscolin, from Viscum coloratum Nakai, in OVA-sensitized mice.

Materials and methods: Female BALB/c mice were sensitized and challenged with ovalbumin (OVA). The mice were randomized into groups and treated with PPE-SVC, viscolin, or rolipram by intraperitoneal injection on 1h before each inhalation of OVA and airway hyperresponsiveness (AHR).

Results: PPE-SVC and viscolin suppressed AHR and reduced eosinophil infiltration of the lungs in OVA-sensitized mice. Moreover, PPE-SVC and viscolin inhibited chemokines, including CCL11 and CCL24, and Th2-associated cytokines in bronchoalveolar lavage fluid. However, PPE-SVC and viscolin could not decrease IL-4, IL-5, and IL-13 levels in cultures of OVA-activated spleen cells.

Conclusion: PPE-SVC and viscolin attenuate airway inflammation and eosinophil infiltration in OVA-sensitized mice.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Asthmatic Agents / isolation & purification
  • Anti-Asthmatic Agents / pharmacology
  • Anti-Asthmatic Agents / therapeutic use*
  • Anti-Inflammatory Agents / isolation & purification
  • Anti-Inflammatory Agents / pharmacology
  • Anti-Inflammatory Agents / therapeutic use*
  • Biphenyl Compounds / isolation & purification
  • Biphenyl Compounds / pharmacology
  • Biphenyl Compounds / therapeutic use*
  • Bronchial Hyperreactivity / immunology
  • Bronchial Hyperreactivity / metabolism
  • Bronchial Hyperreactivity / prevention & control*
  • Bronchoalveolar Lavage Fluid / chemistry
  • Chemokines / metabolism
  • Cytokines / metabolism
  • Eosinophils / metabolism*
  • Female
  • Inflammation / immunology
  • Inflammation / metabolism
  • Inflammation / prevention & control
  • Lung / drug effects
  • Lung / immunology
  • Medicine, Chinese Traditional
  • Mice
  • Mice, Inbred BALB C
  • Ovalbumin
  • Phytotherapy
  • Plant Extracts / chemistry
  • Plant Extracts / pharmacology
  • Plant Extracts / therapeutic use*
  • Propane / analogs & derivatives*
  • Propane / isolation & purification
  • Propane / pharmacology
  • Propane / therapeutic use
  • Random Allocation
  • Rolipram
  • Spleen / cytology
  • Spleen / drug effects
  • Viscum / chemistry*

Substances

  • Anti-Asthmatic Agents
  • Anti-Inflammatory Agents
  • Biphenyl Compounds
  • Chemokines
  • Cytokines
  • Plant Extracts
  • viscolin
  • Ovalbumin
  • Rolipram
  • Propane