Reduced faecal shedding of Escherichia coli O157:H7 in cattle following systemic vaccination with γ-intimin C₂₈₀ and EspB proteins

Daniel A Vilte; Mariano Larzábal; Sergio Garbaccio; Mariela Gammella; Bettina C Rabinovitz; Ana M Elizondo; Rodolfo J C Cantet; Fernando Delgado; Virginia Meikle; Angel Cataldi; Elsa C Mercado

doi:10.1016/j.vaccine.2011.03.079

Reduced faecal shedding of Escherichia coli O157:H7 in cattle following systemic vaccination with γ-intimin C₂₈₀ and EspB proteins

Vaccine. 2011 May 23;29(23):3962-8. doi: 10.1016/j.vaccine.2011.03.079. Epub 2011 Apr 6.

Authors

Daniel A Vilte¹, Mariano Larzábal, Sergio Garbaccio, Mariela Gammella, Bettina C Rabinovitz, Ana M Elizondo, Rodolfo J C Cantet, Fernando Delgado, Virginia Meikle, Angel Cataldi, Elsa C Mercado

Affiliation

¹ Instituto de Patobiología, Instituto Nacional de Tecnología Agropecuaria (INTA), Los Reseros y Las Cabañas, 1712 Castelar, Argentina.

PMID: 21477674
DOI: 10.1016/j.vaccine.2011.03.079

Abstract

Enterohaemorrhagic Escherichia coli (EHEC) O157:H7 is the most prevalent EHEC serotype that has been recovered from patients with haemolytic uremic syndrome (HUS) worldwide. Vaccination of cattle, the main reservoir of EHEC O157:H7, could be a logical strategy to fight infection in humans. This study evaluated a vaccine based on the carboxyl-terminal fragment of 280 amino acids of γ-intimin (γ-intimin C₂₈₀) and EspB, two key colonization factors of E. coli O157:H7. Intramuscular immunization elicited significantly high levels of serum IgG antibodies against both proteins. Antigen-specific IgA and IgG were also induced in saliva, but only the IgA response was significant. Following experimental challenge with E. coli O157:H7, a significant reduction in bacterial shedding was observed in vaccinated calves, compared to control group. These promising results suggest that systemic immunization of cattle with intimin and EspB could be a feasible strategy to reduce EHEC O157:H7 faecal shedding in cattle.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adhesins, Bacterial / administration & dosage
Adhesins, Bacterial / genetics
Adhesins, Bacterial / immunology*
Animals
Antibodies, Bacterial / blood
Antibodies, Bacterial / immunology
Bacterial Outer Membrane Proteins / administration & dosage
Bacterial Outer Membrane Proteins / genetics
Bacterial Outer Membrane Proteins / immunology*
Bacterial Shedding / physiology*
Cattle
Cattle Diseases / immunology
Cattle Diseases / microbiology
Cattle Diseases / prevention & control*
Escherichia coli Infections / immunology
Escherichia coli Infections / microbiology
Escherichia coli Infections / prevention & control
Escherichia coli Infections / veterinary
Escherichia coli O157 / immunology*
Escherichia coli O157 / pathogenicity
Escherichia coli Proteins / administration & dosage
Escherichia coli Proteins / genetics
Escherichia coli Proteins / immunology*
Escherichia coli Vaccines / administration & dosage*
Escherichia coli Vaccines / genetics
Escherichia coli Vaccines / immunology
Feces / microbiology*
Injections, Intramuscular
Male
Recombinant Proteins / administration & dosage
Recombinant Proteins / genetics
Recombinant Proteins / immunology
Treatment Outcome
Vaccination

Substances

Adhesins, Bacterial
Antibodies, Bacterial
Bacterial Outer Membrane Proteins
EaeB protein, E coli
Escherichia coli Proteins
Escherichia coli Vaccines
Recombinant Proteins
eaeA protein, E coli