Genotypic analysis of Enterococci isolated from fecal-polluted water from different sources by pulsed-field gel electrophoresis (PFGE) for application to microbial source tracking

Takashi Furukawa; Hironori Takahashi; Terutoyo Yoshida; Yoshihiro Suzuki

doi:10.1264/jsme2.me10174

Genotypic analysis of Enterococci isolated from fecal-polluted water from different sources by pulsed-field gel electrophoresis (PFGE) for application to microbial source tracking

Microbes Environ. 2011;26(2):181-3. doi: 10.1264/jsme2.me10174. Epub 2011 Feb 26.

Authors

Takashi Furukawa¹, Hironori Takahashi, Terutoyo Yoshida, Yoshihiro Suzuki

Affiliation

¹ Graduate School of Agriculture and Engineering, University of Miyazaki, Miyazaki 889–2192, Japan.

PMID: 21502739
DOI: 10.1264/jsme2.me10174

Abstract

PFGE has potential applications in the source tracking of fecal pollution in aquatic environments. We tried to distinguish the genotypes of Enterococcus faecium collected from fecal-contaminated water using PFGE. Well identified 115 strains of E. faecium were classified into 25 PFGE patterns, and characteristics distinctive to each genotype were recognized. Analysis of the characteristics of genotypes using PFGE can be used to track source of fecal pollution.

MeSH terms

Bacterial Typing Techniques
Base Sequence
DNA, Bacterial / analysis
Electrophoresis, Gel, Pulsed-Field / methods*
Enterococcus faecium / genetics*
Enterococcus faecium / isolation & purification*
Feces
Genetic Variation
Genotype
Japan
Water / chemistry
Water Microbiology*
Water Pollution / analysis

Substances

DNA, Bacterial
Water