Cloning, purification, crystallization and preliminary crystallographic analysis of the human histone deacetylase sirtuin 1

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Apr 1;67(Pt 4):461-3. doi: 10.1107/S1744309111003277. Epub 2011 Mar 25.

Abstract

Human sirtuin 1 is a member of the histone deacetylase family and is involved in cellular aging, tumourigenesis and cellular metabolism. Recombinant sirtuin 1 comprising residues 140-747 was crystallized using the hanging-drop vapour-diffusion method. The crystal diffracted to 3.4 Å resolution and belonged to space group P622, with unit-cell parameters a = b = 203.1, c = 625.3 Å, and is estimated to contain between six and 12 molecules per asymmetric unit.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cloning, Molecular
  • Crystallization
  • Crystallography, X-Ray
  • Humans
  • Sirtuin 1 / chemistry*
  • Sirtuin 1 / genetics
  • Sirtuin 1 / isolation & purification

Substances

  • SIRT1 protein, human
  • Sirtuin 1