[Expression of the MyoD and m-cadherin genes in the myogenic precursor cell culture isolated from muscles of rats at different stages of ontogenesis]

Izv Akad Nauk Ser Biol. 2011 Mar-Apr:(2):145-52.
[Article in Russian]

Abstract

Comparative analysis of expression of the MyoD gene and m-cadherin in the myogenic precursor cells isolated from rats' muscular tissue on the 20th-21st day of embryogenesis, on the third to fifth day of postnatal development, and from adult animals was carried out. No significant differences in expression of the @MyoD@[ital] gene were observed. Nevertheless, we found that in contrast to myogenic cells isolated from postnatal muscular tissues, in the subpopulation of myoblasts isolated from embryonic tissues on the 20th-21st day of embryogenesis cells that were more progressive with respect to differentiation prevailed. These cells demonstrate little proliferative activity alongside with expression of m-cadherin, the protein responsible for cytoadherence, and, as a consequence, a higher rate of differentiation.

Publication types

  • Comparative Study
  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cadherins / biosynthesis
  • Cadherins / genetics*
  • Cell Culture Techniques
  • Cell Differentiation / genetics
  • Cell Proliferation
  • Cells, Cultured
  • Gene Expression Regulation, Developmental*
  • Immunohistochemistry
  • Muscle Development / genetics*
  • Muscle Fibers, Skeletal / metabolism
  • Muscle, Skeletal / embryology
  • Muscle, Skeletal / growth & development
  • Muscle, Skeletal / metabolism*
  • MyoD Protein / biosynthesis
  • MyoD Protein / genetics*
  • Myoblasts / metabolism
  • Polymerase Chain Reaction
  • Rats

Substances

  • Cadherins
  • MyoD Protein
  • M-cadherin