In vitro antitumor activity of silybin nanosuspension in PC-3 cells

Dandan Zheng; Yancai Wang; Dianrui Zhang; Zhaoping Liu; Cunxian Duan; Lejiao Jia; Feihu Wang; Yue Liu; Guangpu Liu; Leilei Hao; Qiang Zhang

doi:10.1016/j.canlet.2011.03.028

In vitro antitumor activity of silybin nanosuspension in PC-3 cells

Cancer Lett. 2011 Aug 28;307(2):158-64. doi: 10.1016/j.canlet.2011.03.028. Epub 2011 Apr 19.

Authors

Dandan Zheng¹, Yancai Wang, Dianrui Zhang, Zhaoping Liu, Cunxian Duan, Lejiao Jia, Feihu Wang, Yue Liu, Guangpu Liu, Leilei Hao, Qiang Zhang

Affiliation

¹ College of Pharmacy, Shandong University, 44 Wenhua Xi Road, Jinan, PR China. [email protected]

PMID: 21507570
DOI: 10.1016/j.canlet.2011.03.028

Abstract

The present study aims to evaluate the antitumor activity of silybin nanosuspension on human prostatic carcinoma PC-3 cell line in vitro. Silybin nanosuspension was prepared by the high pressure homogenization (HPH) method. MTT assay, observation of morphological changes and apoptotic body showed that silybin nanosuspension could significantly enhance the in vitro cytotoxicity against PC-3 cells compared to the silybin solution. Flow cytometric (FCM) analysis demonstrated that silybin nanosuspension induced G1 cycle arrest and apoptosis in PC-3 cells. Thereby, the overall results suggest that the silybin nanosuspension represents a potential source of medicine for the treatment of human prostate cancer.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Apoptosis / drug effects
Cell Line, Tumor
Drug Screening Assays, Antitumor
Flow Cytometry
Humans
In Vitro Techniques
Microscopy, Fluorescence
Silybin
Silymarin / pharmacology*

Substances

Silymarin
Silybin