Abstract
We evaluated the BD GeneOhm MRSA achromopeptidase (ACP) assay, which incorporates a new specimen preparation approach. A total of 1,216 leftover nasal samples were tested; using culture as the gold standard, the sensitivity and specificity were 92% and 94.6%, respectively. The new lysis method provides good sensitivity and simplifies specimen preparation.
Publication types
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Comparative Study
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / genetics
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Bacteriological Techniques / methods*
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Humans
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Methicillin-Resistant Staphylococcus aureus / enzymology
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Methicillin-Resistant Staphylococcus aureus / genetics
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Methicillin-Resistant Staphylococcus aureus / isolation & purification*
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Molecular Diagnostic Techniques / methods*
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Nasal Mucosa / microbiology*
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Polymerase Chain Reaction / methods*
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Reagent Kits, Diagnostic
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Sensitivity and Specificity
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Serine Endopeptidases / genetics*
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Specimen Handling / methods
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Staphylococcal Infections / diagnosis*
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Staphylococcal Infections / microbiology
Substances
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Bacterial Proteins
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Reagent Kits, Diagnostic
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Serine Endopeptidases
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lysyl endopeptidase