Abstract
Methylocella spp. are facultative methanotrophs that grow on methane and multicarbon substrates, such as acetate. Acetate represses transcription of methane monooxygenase of Methylocella silvestris in laboratory culture. DNA stable-isotope probing (DNA-SIP) using (13)C-methane and (12)C-acetate, carried out with Methylocella-spiked peat soil, showed that acetate also repressed methane oxidation by Methylocella in environmental samples.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetates / metabolism*
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Beijerinckiaceae / metabolism*
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Carbon Isotopes / metabolism
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Gene Expression Regulation, Bacterial
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Gene Expression Regulation, Enzymologic*
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Isotope Labeling / methods
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Methane / metabolism*
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Oxidation-Reduction
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Oxygenases / metabolism
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Soil Microbiology*
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Transcription, Genetic
Substances
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Acetates
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Carbon Isotopes
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Oxygenases
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methane monooxygenase
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Methane