Characterization of metallo-beta-lactamase VIM-27, an A57S mutant of VIM-1 associated with Klebsiella pneumoniae ST147

C C Papagiannitsis; S D Kotsakis; E Petinaki; A C Vatopoulos; E Tzelepi; V Miriagou; L S Tzouvelekis

doi:10.1128/AAC.00238-11

Characterization of metallo-beta-lactamase VIM-27, an A57S mutant of VIM-1 associated with Klebsiella pneumoniae ST147

Antimicrob Agents Chemother. 2011 Jul;55(7):3570-2. doi: 10.1128/AAC.00238-11. Epub 2011 Apr 25.

Authors

C C Papagiannitsis¹, S D Kotsakis, E Petinaki, A C Vatopoulos, E Tzelepi, V Miriagou, L S Tzouvelekis

Affiliation

¹ Department of Microbiology, Medical School, University of Athens, Athens, Greece.

Abstract

VIM-27 metallo-β-lactamase, an Ala(57) → Ser variant of VIM-1, was identified in three Klebsiella pneumoniae isolates belonging to sequence type 147. bla(VIM-27) was part of a class 1 integron carried by non-self-transferable plasmids. Kinetic parameters and MIC determinations indicated that VIM-27 hydrolyzed most β-lactams, especially imipenem and cefoxitin, less effectively than VIM-1.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology
Cefoxitin / pharmacology
Drug Resistance, Multiple, Bacterial / genetics
Imipenem / pharmacology
Klebsiella pneumoniae / drug effects
Klebsiella pneumoniae / enzymology*
Klebsiella pneumoniae / genetics
Microbial Sensitivity Tests
Molecular Sequence Data
Molecular Structure
beta-Lactamases / chemistry*
beta-Lactamases / genetics
beta-Lactamases / metabolism*

Substances

Anti-Bacterial Agents
Cefoxitin
Imipenem
VIM-1 metallo-beta-lactamase
beta-Lactamases

Associated data

GENBANK/HQ858608