Abstract
Chemical examination of the methanolic extract from the stem bark of Daphne aurantiaca led to the isolation of three new flavonoids (1-3), and 29 known flavonoids. All 32 compounds were isolated for the first time from Daphne aurantiaca. The isolates were tested for inhibitory activities against lipopolysaccharide (LPS)-induced nitric oxide (NO) production in RAW 264.7 macrophages. Compounds 21 and 24 showed potent inhibitory activities against the production of NO with IC₅₀ values of 0.006 and 0.076 µM, respectively.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Daphne / chemistry*
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Daphne / metabolism
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Flavonoids / chemistry
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Flavonoids / classification
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Flavonoids / isolation & purification*
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Flavonoids / pharmacology*
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Inhibitory Concentration 50
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Lipopolysaccharides / pharmacology
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Macrophages / drug effects*
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Macrophages / metabolism
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Magnetic Resonance Spectroscopy
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Methanol / chemistry
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Nitric Oxide / biosynthesis*
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Nitric Oxide Synthase / antagonists & inhibitors*
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Plant Extracts / chemistry
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Plant Extracts / classification
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Plant Extracts / isolation & purification
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Plant Extracts / pharmacology
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Spectrophotometry, Ultraviolet
Substances
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Flavonoids
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Lipopolysaccharides
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Plant Extracts
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Nitric Oxide
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Nitric Oxide Synthase
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Methanol