Flavonoids from Daphne aurantiaca and their inhibitory activities against nitric oxide production

Chem Pharm Bull (Tokyo). 2011;59(5):653-6. doi: 10.1248/cpb.59.653.

Abstract

Chemical examination of the methanolic extract from the stem bark of Daphne aurantiaca led to the isolation of three new flavonoids (1-3), and 29 known flavonoids. All 32 compounds were isolated for the first time from Daphne aurantiaca. The isolates were tested for inhibitory activities against lipopolysaccharide (LPS)-induced nitric oxide (NO) production in RAW 264.7 macrophages. Compounds 21 and 24 showed potent inhibitory activities against the production of NO with IC₅₀ values of 0.006 and 0.076 µM, respectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Daphne / chemistry*
  • Daphne / metabolism
  • Flavonoids / chemistry
  • Flavonoids / classification
  • Flavonoids / isolation & purification*
  • Flavonoids / pharmacology*
  • Inhibitory Concentration 50
  • Lipopolysaccharides / pharmacology
  • Macrophages / drug effects*
  • Macrophages / metabolism
  • Magnetic Resonance Spectroscopy
  • Methanol / chemistry
  • Nitric Oxide / biosynthesis*
  • Nitric Oxide Synthase / antagonists & inhibitors*
  • Plant Extracts / chemistry
  • Plant Extracts / classification
  • Plant Extracts / isolation & purification
  • Plant Extracts / pharmacology
  • Spectrophotometry, Ultraviolet

Substances

  • Flavonoids
  • Lipopolysaccharides
  • Plant Extracts
  • Nitric Oxide
  • Nitric Oxide Synthase
  • Methanol