Specific detection of antibodies to different flaviviruses using a new immune complex ELISA

Med Microbiol Immunol. 2011 Nov;200(4):233-9. doi: 10.1007/s00430-011-0195-0. Epub 2011 May 1.

Abstract

Immune complex (IC) ELISAs for IgG antibodies to various virus antigens have turned out to be both highly specific and sensitive. During incubation of a labelled antigen with the serum samples, ICs are formed, which bind to microtiter plates coated with rheumatoid factor (RF) IgM. Here, we describe an improved coating of the solid-phase support comparing various Fc-receptor molecules. IC ELISAs were applied to detect human IgG antibodies to the highly virus-specific ED3 domain of West Nile- and tick-borne encephalitis virus envelopes. Compared with other Fc-receptor molecules like RF or C1q, FcγRIIA (CD32) turned out to bind the ICs composed of IgG antibodies and peroxidase-labelled ED3 antigens more efficiently. Due to low background reactions, sera could be tested at a dilution of 1:10. Moreover, using CD32 instead of RF coating, anti-flavivirus antibodies could be detected in various animal species.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology
  • Antibodies, Viral / blood*
  • Antibodies, Viral / immunology
  • Antibody Specificity
  • Antigen-Antibody Complex / immunology
  • Antigens, Viral / immunology
  • Encephalitis Viruses, Tick-Borne / immunology
  • Encephalitis Viruses, Tick-Borne / pathogenicity
  • Encephalitis, Tick-Borne / blood
  • Encephalitis, Tick-Borne / diagnosis*
  • Encephalitis, Tick-Borne / immunology
  • Encephalitis, Tick-Borne / virology
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Fluorescent Antibody Technique, Indirect
  • Humans
  • Immunoglobulin G / blood
  • Immunoglobulin G / immunology
  • Receptors, IgG / immunology
  • Rheumatoid Factor / immunology
  • Sensitivity and Specificity
  • Vaccination
  • Viral Envelope Proteins / immunology
  • West Nile Fever / blood
  • West Nile Fever / diagnosis*
  • West Nile Fever / immunology
  • West Nile Fever / virology
  • West Nile virus / immunology
  • West Nile virus / pathogenicity

Substances

  • Antibodies, Monoclonal
  • Antibodies, Viral
  • Antigen-Antibody Complex
  • Antigens, Viral
  • Immunoglobulin G
  • Receptors, IgG
  • Viral Envelope Proteins
  • glycoprotein E, Flavivirus
  • Rheumatoid Factor