Abstract
In this article, we outline procedures for the dissection of intact kidneys and the isolation of the ureteric bud (UB) and the metanephric mesenchyme (MM) from mouse embryos. The apparatus required for the culture of these tissues in vitro is described in detail as well as the equipment necessary for performing time-lapse imaging studies of the developing kidney.
Publication types
-
Research Support, N.I.H., Extramural
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Dissection / methods*
-
Embryo, Mammalian / anatomy & histology*
-
Embryo, Mammalian / cytology*
-
Kidney / anatomy & histology
-
Kidney / cytology
-
Kidney / embryology*
-
Mice
-
Morphogenesis
-
Time-Lapse Imaging / methods*
-
Ureter / anatomy & histology
-
Ureter / cytology
-
Ureter / embryology