Upgraded Cobas Ampliprep-Cobas TaqMan version 2.0 HIV-1 RNA quantification assay versus first version: correction of underestimations

M Wirden; R Tubiana; S Fourati; M Thevenin; A Simon; A Canestri; Z Ait-Arkoub; C Soulie; A G Marcelin; C Katlama; V Calvez

doi:10.1128/JCM.00061-11

Upgraded Cobas Ampliprep-Cobas TaqMan version 2.0 HIV-1 RNA quantification assay versus first version: correction of underestimations

J Clin Microbiol. 2011 Jul;49(7):2700-2. doi: 10.1128/JCM.00061-11. Epub 2011 May 4.

Authors

M Wirden¹, R Tubiana, S Fourati, M Thevenin, A Simon, A Canestri, Z Ait-Arkoub, C Soulie, A G Marcelin, C Katlama, V Calvez

Affiliation

¹ Department of Virology, Pitié-Salpêtriere Hospital, Paris, France. [email protected]

Abstract

The large underestimations of HIV RNA quantification observed in 17 patients with the first version of Cobas TaqMan assay have been successfully corrected in the upgraded version 2.0. In comparison with the Abbott RealTime assay, the mean difference that was 1.18 log(10) copies/ml is now zero. The discrepancies have disappeared.

Publication types

Comparative Study
Evaluation Study

MeSH terms

HIV Infections / virology*
HIV-1 / genetics
HIV-1 / isolation & purification*
Humans
RNA, Viral / genetics
RNA, Viral / isolation & purification*
Reagent Kits, Diagnostic*
Viral Load / methods*

Substances

RNA, Viral
Reagent Kits, Diagnostic