Structure of CBM4 from Clostridium thermocellum cellulase K

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 May 1;67(Pt 5):527-30. doi: 10.1107/S1744309111003307. Epub 2011 Apr 20.

Abstract

Here, a 2.0 Å resolution X-ray structure of Clostridium thermocellum cellulase K family 4 carbohydrate-binding module (CelK CBM4) is reported. The resulting structure was refined to an R factor of 0.212 and an R(free) of 0.274. Structural analysis shows that this new structure is very similar to the previously solved structure of C. thermocellum CbhA CBM4. Most importantly, these data support the previously proposed notion of an extended binding pocket using a novel tryptophan-containing loop that may be highly conserved in clostridial CBM4 proteins.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Cellulase / chemistry*
  • Clostridium thermocellum / enzymology*
  • Crystallography, X-Ray
  • Models, Molecular
  • Protein Structure, Secondary
  • Protein Structure, Tertiary

Substances

  • Cellulase

Associated data

  • PDB/3P6B