Studies on the development of an ELISA kit for microbiological monitoring. 2. Improvement of the prototype ELISA kit with special references to mouse hepatitis virus antigen

N Kagiyama; A Takakura; E Terada; Y Sakurai; H Suzuki

doi:10.1538/expanim1978.39.1_97

Studies on the development of an ELISA kit for microbiological monitoring. 2. Improvement of the prototype ELISA kit with special references to mouse hepatitis virus antigen

Jikken Dobutsu. 1990 Jan;39(1):97-102. doi: 10.1538/expanim1978.39.1_97.

Authors

N Kagiyama¹, A Takakura, E Terada, Y Sakurai, H Suzuki

Affiliation

¹ Department of Laboratory Animal Medicine, Central Institute for Experimental Animals, Kawasaki-shi, Japan.

PMID: 2154386
DOI: 10.1538/expanim1978.39.1_97

Abstract

Improvement of the mouse hepatitis virus (MHV) antigen in a prototype ELISA kit was performed. Equivalent divalent antigens of MHV Nu-67 and S strains with a protein concentration of 10 micrograms/ml showed the best sensitivity and specificity for the detection of MHV and sialodacryoadenitis/Parker's rat coronavirus antibodies in mice and rats, respectively. An increase in the reliability of macroscopic evaluation of both antibody tests is expected by using the newly manufactured kit with the improved antigen.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Animals, Laboratory / immunology*
Antigens, Viral / analysis*
Enzyme-Linked Immunosorbent Assay / methods*
Hepatitis Antibodies / analysis
Hepatitis, Viral, Animal / diagnosis
Mice
Murine hepatitis virus / immunology*
Reagent Kits, Diagnostic / standards*
Rodent Diseases / diagnosis

Substances

Antigens, Viral
Hepatitis Antibodies
Reagent Kits, Diagnostic