Triggered activity of a nicking endonuclease for mercuric(II) ion-mediated duplex-like DNA cleavage

Feng Li; Yan Feng; Shufeng Liu; Bo Tang

doi:10.1039/c1cc11858g

Triggered activity of a nicking endonuclease for mercuric(II) ion-mediated duplex-like DNA cleavage

Chem Commun (Camb). 2011 Jun 14;47(22):6347-9. doi: 10.1039/c1cc11858g. Epub 2011 May 6.

Authors

Feng Li¹, Yan Feng, Shufeng Liu, Bo Tang

Affiliation

¹ College of Chemistry, Chemical Engineering and Materials Science, Engineering Research Center of Pesticide and Medicine Intermediate Clean Production, Ministry of Education, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Normal University, Jinan 250014, China.

PMID: 21547294
DOI: 10.1039/c1cc11858g

Abstract

The cleavage activity of a nicking endonuclease towards metal-ion-mediated duplex-like DNA can be triggered by the corresponding metal ions, which was demonstrated with mercuric(II) ion as a model via a simple electrochemical protocol.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Catalysis
DNA / metabolism*
DNA Cleavage*
Deoxyribonuclease I / metabolism
Deoxyribonucleases, Type II Site-Specific / metabolism*
Electrochemical Techniques
Electrodes
Gold / chemistry
Ions / chemistry
Mercury / chemistry*

Substances

Ions
Gold
DNA
Deoxyribonuclease I
Deoxyribonucleases, Type II Site-Specific
GGATC-specific type II deoxyribonucleases
Mercury