Morphologic and mitochondrial characterization of human spermatogenic cells dispersed in wet preparation for testicular sperm extraction: establishment of a microscopic diagram of developing human spermatogenic cells

Hidenobu Okuda; Akira Tsujimura; Keisuke Yamamoto; Shinichiro Fukuhara; Jiro Nakayama; Hiroshi Kiuchi; Tetsuya Takao; Yasushi Miyagawa; Norio Nonomura; Akihiko Okuyama

doi:10.1016/j.fertnstert.2011.04.026

Morphologic and mitochondrial characterization of human spermatogenic cells dispersed in wet preparation for testicular sperm extraction: establishment of a microscopic diagram of developing human spermatogenic cells

Fertil Steril. 2011 Jun 30;95(8):2665-8. doi: 10.1016/j.fertnstert.2011.04.026. Epub 2011 May 5.

Authors

Hidenobu Okuda¹, Akira Tsujimura, Keisuke Yamamoto, Shinichiro Fukuhara, Jiro Nakayama, Hiroshi Kiuchi, Tetsuya Takao, Yasushi Miyagawa, Norio Nonomura, Akihiko Okuyama

Affiliation

¹ Department of Urology, Osaka University Graduate School of Medicine, Osaka, Japan.

PMID: 21549365
DOI: 10.1016/j.fertnstert.2011.04.026

Abstract

The successful identification of spermatozoa at microdissection testicular sperm extraction is critical and can depend on the judgment of experienced reproductive clinicians. Therefore, we characterized human spermatogenic cells based on stage-specific mitochondrial location and morphologic change by using a vital mitochondrion-specific fluorescent probe, Mitotracker, and stage-specific antibodies to make a useful microscopic diagram.

MeSH terms

Cell Shape*
Fluorescent Antibody Technique
Fluorescent Dyes
Humans
Male
Microdissection*
Microscopy, Fluorescence* / methods
Mitochondria / ultrastructure*
Organic Chemicals
Sperm Retrieval*
Spermatogenesis*
Spermatozoa / ultrastructure*
Testis / cytology
Testis / ultrastructure*

Substances

Fluorescent Dyes
Organic Chemicals
red dye CMXRos