Combining quantitative proteomics data processing workflows for greater sensitivity

Nat Methods. 2011 Jun;8(6):481-3. doi: 10.1038/nmeth.1604. Epub 2011 May 8.

Abstract

We here describe a normalization method to combine quantitative proteomics data. By merging the output of two popular quantification software packages, we obtained a 20% increase (on average) in the number of quantified human proteins without suffering from a loss of quality. Our integrative workflow is freely available through our user-friendly, open-source Rover software (http://compomics-rover.googlecode.com/).

MeSH terms

  • Algorithms
  • Computational Biology
  • Databases, Protein
  • Electronic Data Processing / methods*
  • Humans
  • Proteomics / standards
  • Proteomics / statistics & numerical data*
  • Quality Control
  • Software*
  • Workflow