Microsatellite loci isolation in the Canarian common chaffinch (Fringilla coelebs) and their utility in other Canarian finches

N M Suárez; E Betancor; J J Pestano

doi:10.1111/j.1755-0998.2009.02599.x

Microsatellite loci isolation in the Canarian common chaffinch (Fringilla coelebs) and their utility in other Canarian finches

Mol Ecol Resour. 2009 Jul;9(4):1164-6. doi: 10.1111/j.1755-0998.2009.02599.x. Epub 2009 Feb 26.

Authors

N M Suárez¹, E Betancor, J J Pestano

Affiliation

¹ Departamento de Genética, Facultad de Medicina, Universidad de Las Palmas de Gran Canaria, Las Palmas, 35080 Gran Canaria, Spain.

PMID: 21564863
DOI: 10.1111/j.1755-0998.2009.02599.x

Abstract

The taxonomic classification of the common chaffinch (Fringilla coelebs) that inhabits the Canary Islands has been under debate for decades, mainly due to the absence of nuclear DNA analyses. In this study we describe the isolation and characterization of ten microsatellite loci (AAAG, AAAT and GT) from a La Palma specimen using an enrichment protocol. Two loci were monomorphic in the populations analysed (La Gomera and La Palma), but the remaining ones presented 2 or more alleles, with an average of 11.63 alleles per locus and an average observed heterozygosity of 0.735 (n = 44). All loci were tested for their utility in other Canarian populations and other finch species.