Development of 10 microsatellite loci in the ling (Molva molva)

Anna-Karin Ring; Halvor Knutsen; David Fiani; A R Hoelzel; Carl André

doi:10.1111/j.1755-0998.2009.02677.x

Development of 10 microsatellite loci in the ling (Molva molva)

Mol Ecol Resour. 2009 Sep;9(5):1401-3. doi: 10.1111/j.1755-0998.2009.02677.x. Epub 2009 Mar 31.

Authors

Anna-Karin Ring¹, Halvor Knutsen, David Fiani, A R Hoelzel, Carl André

Affiliation

¹ Department of Marine Ecology - Tjärnö, University of Gothenburg, S-462 96 Strömstad, Sweden Institute of Marine Research, Flødevigen Marine Research Station, N-4817 His, Norway School of Biological and Biomedical Sciences, Durham University, South Road, Durham DH1 3LE, UK.

PMID: 21564919
DOI: 10.1111/j.1755-0998.2009.02677.x

Abstract

We developed primers for two dinucleotide and eight tetranucleotide microsatellite loci in a marine fish, the ling (Molva molva). All markers were obtained from partial genomic DNA libraries and characterized in 55 unrelated individuals from one putative population. The number of alleles ranged from five to 24 (average 10.5) per locus, and the observed heterozygosity ranged from 0.218 to 0.981 (average 0.643). No loci amplified in two other gadoid species tested, the Atlantic cod (Gadus morhua) and the tusk (Brosme brosme).