Abstract
A new ligand and two ruthenium(II) complexes [Ru(bpy)(2)(DNPIP)](ClO(4))(2)1 and [Ru(bpy)(2)(DAPIP)](ClO(4))(2)2 were synthesized and characterized. The DNA-binding constants for complexes 1 and 2 were determined to be 2.24 (±0.30) × 10(5) M(-1) (s = 1.29) and 6.34 (±0.32) × 10(4) M(-1) (s = 2.84). The photocleavage of pBR322 DNA by Ru(II) complexes was investigated. The cytotoxicity of complexes 1 and 2 were assessed against three tumor cell lines. The apoptosis and cellular uptake were studied. The retardation assay of pGL 3 plasmid DNA was explored. The cell cycle arrest was analysized by flow cytometry. The antioxidant activities of the ligand and complexes were also investigated.
Crown Copyright © 2011. Published by Elsevier Masson SAS. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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2,2'-Dipyridyl / chemistry
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2,2'-Dipyridyl / metabolism
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2,2'-Dipyridyl / pharmacology*
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Antineoplastic Agents / chemical synthesis
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Antineoplastic Agents / pharmacology*
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Antioxidants / chemical synthesis
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Antioxidants / pharmacology
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Apoptosis / drug effects
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Cell Cycle / drug effects*
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Cell Line, Tumor
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Coordination Complexes / chemical synthesis
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Coordination Complexes / pharmacology*
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DNA / chemistry
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DNA / metabolism
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DNA Cleavage / drug effects
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Differential Thermal Analysis
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Electrophoretic Mobility Shift Assay
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Flow Cytometry
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Humans
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Intercalating Agents / chemical synthesis
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Intercalating Agents / pharmacology*
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Ligands
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Liver Neoplasms / drug therapy*
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Liver Neoplasms / pathology
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Phenanthrolines / chemistry
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Phenanthrolines / metabolism
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Phenanthrolines / pharmacology*
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Plasmids
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Ruthenium / chemistry
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Ruthenium / metabolism
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Ruthenium / pharmacology*
Substances
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Antineoplastic Agents
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Antioxidants
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Coordination Complexes
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Intercalating Agents
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Ligands
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Phenanthrolines
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2,2'-Dipyridyl
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Ruthenium
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DNA
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calf thymus DNA