Process evaluations and economic analyses of recombinant human lysozyme and hen egg-white lysozyme purifications

Biotechnol Prog. 2011 May-Jun;27(3):733-43. doi: 10.1002/btpr.593. Epub 2011 May 13.

Abstract

Human lysozyme and hen egg-white lysozyme have antibacterial, antiviral, and antifungal properties with numerous potential commercial applications. Currently, hen egg-white lysozyme dominates low cost applications but the recent high-level expression of human lysozyme in rice could provide an economical source of lysozyme. This work compares human lysozyme and hen egg-white lysozyme adsorption to the cation exchange resin, SP-Sepharose FF, and the effect of rice extract components on lysozyme purification. With one exception, the dynamic binding capacities of human lysozyme were lower than those of hen egg-white at pH 4.5, 6, and 7.5 with ionic strengths ranging from 0 to 100 mM (5-20 mS). Ionic strength and pH had a similar effect on the adsorption capacities, but human lysozyme was more sensitive to these two factors than hen egg-white lysozyme. In the presence of rice extract, the dynamic binding capacities of human and hen egg-white lysozymes were reduced by 20-30% and by 32-39% at pH 6. Hen egg-white lysozyme was used as a benchmark to compare the effectiveness of human lysozyme purification from transgenic rice extract. Process simulation and cost analyses for human lysozyme purification from rice and hen egg-white lysozyme purification from egg-white resulted in similar unit production costs at 1 ton per year scale.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adsorption
  • Animals
  • Cation Exchange Resins
  • Costs and Cost Analysis
  • Humans
  • Muramidase / economics
  • Muramidase / isolation & purification*
  • Oryza / genetics
  • Protein Binding
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / economics
  • Recombinant Proteins / isolation & purification*

Substances

  • Cation Exchange Resins
  • Recombinant Proteins
  • hen egg lysozyme
  • Muramidase