Rubrimonas shengliensis sp. nov. and Polymorphum gilvum gen. nov., sp. nov., novel members of Alphaproteobacteria from crude oil contaminated saline soil

Syst Appl Microbiol. 2011 Jul;34(5):321-7. doi: 10.1016/j.syapm.2011.03.004. Epub 2011 May 19.

Abstract

Four bacterial strains were isolated from a crude oil contaminated saline soil in Shengli Oilfield, China. Strains SL014B-28A2(T) and SL014B-80A1 were most closely related to Rubrimonas cliftonensis OCh 317(T), while strains SL003B-26A1(T) and SL003B-26A2 were most closely related to but readily different from the species in the Pannonibacter-Labrenzia-Roseibium-Stappia cluster. The major fatty acids were C(18:1)ω7c, C(16:0), C(18:0) and 11-Methyl C(18:1)ω7c, and C(18:1)ω7c, 11-Methyl C(18:1)ω7c and C(18:0), respectively, for these two groups of isolates. Q-10 was the predominant ubiquinone. The G+C contents of genomic DNA of the four isolates were 67.9, 69.7, 65.6 and 65.6 mol%. Based on the polyphasic taxonomic characteristics, strains SL014B-28A2(T) and SL014B-80A1 represented a novel species of the genus Rubrimonas, for which the name Rubrimonas shengliensis sp. nov. is proposed, with strain SL014B-28A2(T) (=LMG 26072(T)=CGMCC 1.9170(T)) as the type strain. Isolates SL003B-26A1(T) and SL003B-26A2 represented a novel genus and species of the family Rhodobacteraceae, for which the name Polymorphum gilvum gen. nov., sp. nov. is proposed, with strain SL003B-26A1(T) (=LMG 25793(T)=CGMCC 1.9160(T)) as the type strain.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Composition
  • China
  • DNA, Bacterial / genetics
  • Fatty Acids / analysis*
  • Genes, Bacterial*
  • Genes, rRNA
  • Lipids / analysis
  • Molecular Sequence Data
  • Phylogeny*
  • RNA, Ribosomal, 16S / genetics
  • Rhodobacteraceae / classification*
  • Rhodobacteraceae / genetics
  • Rhodobacteraceae / isolation & purification
  • Rhodobacteraceae / physiology
  • Soil Microbiology*
  • Ubiquinone / analysis

Substances

  • DNA, Bacterial
  • Fatty Acids
  • Lipids
  • RNA, Ribosomal, 16S
  • Ubiquinone

Associated data

  • GENBANK/GU125651
  • GENBANK/GU125652
  • GENBANK/GU125654
  • GENBANK/GU125655