Abstract
Here, we describe a route orthogonal gene expression which combines orthogonal transcription and translation using library-based selections. We show how orthogonal gene expression can be used to create a minimal orthogonal ribosome and describe how to create orthogonal transcription-translation feed forward loops that introduce tailored information processing delays into gene expression.
Copyright © 2011 Elsevier Inc. All rights reserved.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
DNA-Directed RNA Polymerases / genetics
-
DNA-Directed RNA Polymerases / metabolism
-
Escherichia coli / genetics*
-
Escherichia coli / metabolism*
-
Escherichia coli Proteins / genetics
-
Escherichia coli Proteins / metabolism*
-
Gene Expression Regulation, Bacterial*
-
Gene Library
-
Nucleic Acid Conformation
-
Promoter Regions, Genetic
-
Protein Biosynthesis
-
RNA, Messenger / chemistry
-
RNA, Messenger / genetics
-
RNA, Messenger / metabolism
-
Ribosomes / genetics
-
Ribosomes / metabolism
-
Transcription, Genetic
-
Viral Proteins / genetics
-
Viral Proteins / metabolism
Substances
-
Escherichia coli Proteins
-
RNA, Messenger
-
Viral Proteins
-
bacteriophage T7 RNA polymerase
-
DNA-Directed RNA Polymerases