Orthogonal gene expression in Escherichia coli

Methods Enzymol. 2011:497:115-34. doi: 10.1016/B978-0-12-385075-1.00005-6.

Abstract

Here, we describe a route orthogonal gene expression which combines orthogonal transcription and translation using library-based selections. We show how orthogonal gene expression can be used to create a minimal orthogonal ribosome and describe how to create orthogonal transcription-translation feed forward loops that introduce tailored information processing delays into gene expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA-Directed RNA Polymerases / genetics
  • DNA-Directed RNA Polymerases / metabolism
  • Escherichia coli / genetics*
  • Escherichia coli / metabolism*
  • Escherichia coli Proteins / genetics
  • Escherichia coli Proteins / metabolism*
  • Gene Expression Regulation, Bacterial*
  • Gene Library
  • Nucleic Acid Conformation
  • Promoter Regions, Genetic
  • Protein Biosynthesis
  • RNA, Messenger / chemistry
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Ribosomes / genetics
  • Ribosomes / metabolism
  • Transcription, Genetic
  • Viral Proteins / genetics
  • Viral Proteins / metabolism

Substances

  • Escherichia coli Proteins
  • RNA, Messenger
  • Viral Proteins
  • bacteriophage T7 RNA polymerase
  • DNA-Directed RNA Polymerases